Ettan dige imager user manual

Ettan DIGE system workflow 14 Measurement of variation 15 Experimental design 16 Protocol 21 1. Introduction 23 2. Preparation of a cell lysate compatible Variable Mode Imager • Ettan DALT Gel system can be found in the Ettan DIGE System User Manual (code ). This manual is also available on the Amersham Biosciences. Typhoon imagers are fully optimized as part of the Ettan DIGE system, and seamless integration with DeCyder Differential Analysis Software is ensured for all models. Special tray templates that are part of the latest scanner control software and new optional Gel Alignment Guides add higher throughput and ease of handling 2-D gels run on Ettan. Ettan™ DIGE applications are described in detail in the Ettan™ DIGE user manual. E. Separating proteins in the second dimension. Low-fluorescence glass plates must be used for gels used in Ettan™ DIGE system. Standard glass or plastic-backed plates can result in the generation of a high background signal.

Two-dimensional difference gel electrophoresis (2D DIGE) is a modified form of 2D electrophoresis (2D E) that allows one to compare two or three protein samples simultaneously on the same gel. Ettan™ DIGE Imager (GE Healthcare) Green (/25 nm) light source Orange (/25 nm) filter For multiplex applications, violet excitation filter (/20 nm) and orange emission filter will avoid cross talk with Cy2 and Cy3 Table 3. Recommended Imaging Conditions for Different Imaging Systems. Ettan DIGE enabled The multiplexing capability of Ettan 2-D fluorescence difference gel electrophoresis (DIGE) enables full standardization of 2-D results (Fig 9).Typhoon is one of the only imagers fully tested to meet this application's requirements for sensitivity, linearity, and accuracy.

Difference Gel Electrophoresis (2-D DIGE) analysis and is a key element in the DIGE system. Unlike other 2-D electrophoresis methods, DIGE allows the option of reference to an internal standard for each spot, effectively eliminating gel-to-gel variation and delivering highly accurate, reproducible, and dependable quantitation. system can be found in the Ettan DIGE System User Manual (code ). This manual is also available on the GE Healthcare website (www.doorway.ru). Dye characteristics CyDye DIGE Fluor Cy3 saturation dye Molecular formula C 37 H 44 N 4 O 6 S Formula weight Absorption max (in DMF) ± 3 nm Emission max (in DMF) ± 5 nm. Figure 2 shows a typical gel image produced with the Ettan DIGE Imager. Ordering information Ettan DIGE Imager, including installation kit (power cable, CD with scan control software, test slides, and user manual with installation guidelines) Ettan DIGE Imager Cassette, x mm, for DALT gel sandwiches Ettan DIGE.